Introduction: This study was conducted to compare three rotary files of Superfile III Denco, Sp1 Gold v taper, and ProTaper in terms of debris extrusion to find the best treatment option. Methods: In total, 51 extracted mandibular premolars were randomly assigned to three groups (n=17). Rotary systems of Sp1 Gold v taper, Superfile III Denco, and ProTaper universal were used in the first, second, and third groups, respectively. Canals were prepared up to the F2 file, and this process was conducted only by one operator. Moreover, an Orifice shaper specific to each group was used first, and files were removed from canals and then cleaned by wet gauze. In the preparation procedure, canals were washed to extract all debris from the canal. Eppendorf tubes were used to collect debris. Results: Mean debris values extruded from canals in the Sp1 gold, Superfile Denco, and ProTaper universal files were 0. 0025, 0. 0008, and 0. 0014, respectively. There was no significant difference among debris extruded by Superfile Denco III, SP1 gold V taper, and ProTaper universal files (P<0. 05). Conclusions: All instrumentation systems cause the extrusion of debris to the apical area. According to the results of the present study, there was no significant difference among debris extruded by Superfile Denco, SP1 Gold, and ProTaper universal files. It should be mentioned that because of variations in the study designs, the direct comparison of different studies that addressed this problem is not possible, and in vivo studies should be performed to evaluate its clinical relevance.